Search results for " endometriosis"

showing 10 items of 39 documents

Single-cell RNA sequencing of oocytes from ovarian endometriosis patients reveals a differential transcriptomic profile associated with lower quality

2018

Abstract STUDY QUESTION Do oocytes from women with ovarian endometriosis (OE) have a different transcriptomic profile than those from healthy women? SUMMARY ANSWER Oocytes from endometriosis patients, independently of whether they came from the affected ovary, exhibited a differential transcriptomic profile compared to oocytes from healthy egg donors. WHAT IS KNOWN ALREADY Studies of endometriosis have sought to determine whether OE affects oocyte quality. While many reports indicate that oocytes recovered from endometriotic ovaries may be affected by the disease, other studies have found no significant differences among oocyte/embryo quality and fertilization, implantation and pregnancy ra…

Adult0301 basic medicineAdolescentEndometriosisEndometriosisOvaryBiologyAndrologyTranscriptomeYoung Adult03 medical and health sciences0302 clinical medicineHuman fertilizationmedicineHumansOvarian Diseases030219 obstetrics & reproductive medicineSequence Analysis RNAGene Expression ProfilingRehabilitationEmbryogenesisObstetrics and Gynecologymedicine.diseaseOocyte030104 developmental biologymedicine.anatomical_structureReproductive MedicineCase-Control StudiesOocytesOvarian EndometriosisFemaleSingle-Cell AnalysisTranscriptomeEmbryo qualityHuman Reproduction
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Immunohistochemical Investigation of Metastasis-Related Chemokines in Deep-Infiltrating Endometriosis and Compromised Pelvic Sentinel Lymph Nodes

2015

Endometriosis is a prevalent benign disease, despite sharing several similarities with malignancies, such as the possibility of lymphatic spread. In malignancies, chemokines play a sovereign role in the process of metastasis. Metastasis-related chemokine axes have not yet been assessed in deep-infiltrating endometriosis (DIE), and this investigation was the aim of our study. The expression of these chemokines was investigated by immunohistochemistry in rectovaginal DIE lesions and in matched pelvic sentinel lymph nodes (PSLNs) of patients with endometriosis (n = 27), and their expression in the eutopic endometrium (EE) of endometriosis-free women (n = 20) was used as controls. Their stainin…

AdultChemokinePathologymedicine.medical_specialtyEndometriosisEndometriosislymphatic spreadMetastasisEndometriumYoung AdultCell MovementLymphatic SpreadmedicineHumansEutopic endometriumpelvic sentinel lymph nodeRetrospective StudiesbiologySentinel Lymph Node Biopsybusiness.industrychemokineendometriosiObstetrics and GynecologyMiddle Agedmedicine.diseaseImmunohistochemistryDeep infiltrating endometriosisbiology.proteinImmunohistochemistryFemaleLymph NodesLymphChemokinesbusinessBiomarkersReproductive Sciences
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Delayed Coloanal Anastomosis for rectovaginal fistula after colorectal resection for deep endometriosis

2016

The deep infiltrating endometriosis, defined as a subperitoneal infiltration of endometrial implants of ≥ 5 mm involving not only the colorectal tract but also rectovaginal septum, vagina and bladder often requires a challenging surgery. Endometriosis nodes of the rectovaginal septum, if symptomatic, need a resection of the involved colorectal tract with colorectal or coloanal anastomosis. Unfortunately in these cases is not uncommon the possibility of a postoperative rectovaginal fistula (RVF), caused by the weakness of the septum that must be skeletonized to completely remove the endometriosis nodes. Here we present a case of anastomotic leakage with high RVF after colorectal resection an…

AdultColonic DiseaseTime FactorsEndometriosisdeep endometriosisAnastomotic LeakPostoperative HemorrhageColonic DiseasesPostoperative ComplicationsHumansBlood TransfusionEndometriosiSigmoid Diseasesdelayed coloanal anastomosisIleostomyPelvic InfectionAnastomosis SurgicalRectal DiseaseSigmoid Diseaserectovaginal fistulaPleural EffusionRectal Diseasesdeep endometriosis; delayed coloanal anastomosis; rectovaginal fistulaFemalePostoperative ComplicationHuman
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Robotic treatment of colorectal endometriosis: technique, feasibility and short-term results

2012

background: Deep infiltrating endometriosis (DIE) is a complex disease that impairs the quality of life and the fertility of women. Since a medical approach is often insufficient, a minimally invasive approach is considered the gold standard for complete disease excision. Roboticassisted surgery is a revolutionary approach, with several advantages compared with traditional laparoscopic surgery. methods: From March 2010 to May 2011, we performed 22 consecutive robotic-assisted complete laparoscopic excisions of DIE endometriosis with colorectal involvement. All clinical data were collected by our team and all patients were interviewed preoperatively and 3 and 6 months post-operatively and ye…

AdultLaparoscopic surgerymedicine.medical_specialtyAdolescentmedicine.medical_treatmentEndometriosisEndometriosisIleostomyPostoperative ComplicationsROBOTIC SURGERYLaparotomymedicineHumansLaparoscopyRetrospective Studiesmedicine.diagnostic_testbusiness.industryRehabilitationColostomyObstetrics and GynecologyRoboticsMiddle AgedDebulkingmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaSurgeryRectal DiseasesTreatment OutcomeReproductive MedicineFemaleLaparoscopySegmental resectionbusinessdeep endometriosis / colorectal endometriosis / robotic-assisted laparoscopy
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The gene expression profile of cumulus cells reveals altered pathways in patients with endometriosis

2014

PURPOSE: The objective of this experimental study was to compare the global gene expression profile of CC of mature oocytes in 18 patients with severe endometriosis and CC in 18 control patients affected by a severe male factor. METHODS: For each group, the CC were pooled, RNA was extracted and a microarray performed. For validating the microarray, a quantitative real-time PCR was performed in the CC of an independent set of patients with endometriosis (n = 5) and controls (n = 7). RESULTS: 595 differentially expressed genes (320 down-regulated, 275 up-regulated, p < 0.05, fold change ≥1.5) were identified. The most significant changes were observed in genes involved in the chemokine signal…

AdultMaleAdolescentMicroarrayEndometriosisEndometriosisDown-Regulationmacromolecular substancesBiologyBioinformaticsTranscriptomeAndrologyYoung AdultDownregulation and upregulationSettore BIO/13 - Biologia ApplicataGene expressionGeneticsmedicineHumansGenetics (clinical)Cumulus CellsMicroarray analysis techniquesGene Expression Profilingmusculoskeletal neural and ocular physiologygene expression profile cumulus cellObstetrics and GynecologyGeneral Medicinegene expression profile cumulus cells; microarray; EndometriosisMicroarray Analysismedicine.diseaseUp-RegulationGene expression profilingReproductive Physiology and Diseasenervous systemReproductive MedicineCase-Control StudiesOocytesFemaleSignal transductionTranscriptomemicroarraySignal TransductionDevelopmental Biology
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Eutopic endometrium from women with endometriosis does not exhibit neurotrophic properties

2012

The role of neurotrophins in eutopic endometrium from endometriosis-patients was investigated in a prospective study using immunofluorescence-staining, Western blot and a neuronal growth assay. The nerve growth factor is expressed in primary endometrial cell culture from women with and without endometriosis. Western blot analysis of endometrial biopsies or uterine fluid from patients with and without endometriosis shows no difference in the neurotrophin expression. We could not find a difference between patients with and without endometriosis with regards to the neurite outgrowth of sensory ganglia when treated with conditioned cultured medium or uterine fluid. This result refutes the assum…

AdultPathologymedicine.medical_specialtyNeuriteBlotting WesternImmunologyEndometriosisEndometriosisFluorescent Antibody TechniqueNeuriteEndometrial innervationAndrologyYoung AdultEndometrial innervation; Endometriosis; Pelvic painPelvic painWestern blotNeurotrophic propertieNerve Growth FactorNeuritesmedicineHumansImmunology and AllergyEutopic endometriumEndometriosiProspective cohort studyUterine Diseasesbiologymedicine.diagnostic_testbusiness.industryPelvic painDysmenorrhoeaMiddle Agedmedicine.diseaseSettore MED/40 - Ginecologia E OstetriciaNerve growth factorNeurologyCulture Media Conditionedbiology.proteinUterine DiseaseFemaleNeurology (clinical)medicine.symptombusinessNeurotrophinHuman
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Renal endometriosis mimicking complicated cysts of kidney: report of two cases

2017

Endometriosis is a common gynecologic disorder characterized by ectopic endometrial tissue growth outside the uterine cavity. Although usually occurring in pelvic organs, endometrial lesions may involve urinary tract. Renal endometriosis is extremely rare and it has only occasionally been reported in the past. We report two cases of patients with renal cystic lesions, incidentally found at imaging techniques during oncologic follow-up for gastric sarcoma and melanoma, initially misinterpreted as complicated haemorrhagic cysts and then histologically characterized as renal localizations of extragenital endometriosis.

AdultPathologymedicine.medical_specialtyUrinary systemEndometriosisEndometriosis030218 nuclear medicine & medical imagingClinical PracticeDiagnosis DifferentialRenal endometriosis - Extragenital endometriosis - Kidney - Complicated renal cyst - Endometrioma - Shading sign03 medical and health sciences0302 clinical medicinemedicineHumansPelvic organKidney030219 obstetrics & reproductive medicinebusiness.industryMelanomaKidney Diseases Cysticmedicine.diseaseEctopic endometrial tissueSettore MED/18 - Chirurgia Generalemedicine.anatomical_structureFemaleKidney DiseasesSarcomaUterine cavitybusinessSettore MED/36 - Diagnostica Per Immagini E Radioterapia
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Long-term evaluation of quality of life and gastrointestinal well-being after segmental colo-rectal resection for deep infiltrating endometriosis (EN…

2019

Purposes: The primary objective is to assess the long-term quality of life (QoL) and gastrointestinal well-being in patients with endometriosis (DIE) who underwent segmental resection (SR), through specific questionnaires focused on endometriosis and specific gastrointestinal evaluation. The secondary objectives are represented by the evaluation of peri-operative and post-operative outcomes of the procedure. Methods: This observational cohort study ENDO-RESECT (ClinicalTrials.gov ID: NCT03824054) reports all clinical data about women who underwent SR for DIE between October 2005 and November 2017. In the part of the study dedicated to the QoL assessment, the questionnaires adopted were the …

AdultQuality of lifemedicine.medical_specialtyAbdominal painConstipationAdolescentGastrointestinal DiseasesEndometriosisEndometriosisSegmental colo-rectal resectionHospital Anxiety and Depression ScaleGastrointestinal symptomsCohort StudiesYoung AdultPostoperative ComplicationsQuality of lifePregnancySurveys and QuestionnairesInternal medicineGastrointestinal symptommedicineHumansDeep infiltrating endometriosis; Gastrointestinal symptoms; Intestinal endometriosis; Personalized medicine; Quality of life; Segmental colo-rectal resection; Adolescent; Adult; Cohort Studies; Colorectal Neoplasms; Endometriosis; Female; Gastrointestinal Diseases; Humans; Middle Aged; Postoperative Complications; Pregnancy; Quality of Life; Surveys and Questionnaires; Treatment Outcome; Young Adultbusiness.industryIntestinal endometriosiObstetrics and GynecologyGeneral MedicineMiddle Agedmedicine.diseasePersonalized medicineDeep infiltrating endometriosiTreatment OutcomeSettore MED/40 - GINECOLOGIA E OSTETRICIADeep infiltrating endometriosisDeep infiltrating endometriosis; Intestinal endometriosis; Segmental colo-rectal resection; Quality of life; Gastrointestinal symptoms; Personalized medicineDefecationFemaleIntestinal endometriosisSegmental resectionmedicine.symptomColorectal NeoplasmsbusinessCohort study
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Primary umbilical endometrioma: Analyzing the pathogenesis of endometriosis from an unusual localization

2015

Abstract Objective This report presents a rare case of symptomatic primary umbilical endometriosis and reviews the literature on the topic with the aim to clarify some questions on the origin of endometriosis. Case report A 33-year-old woman with cyclic umbilical bleeding was found to have umbilical endometriosis. She had no history of pelvic or abdominal surgery. There was no past history of endometriosis or endometriosis-associated symptoms. An omphalectomy was performed after explorative laparoscopy to carefully inspect the abdominopelvic cavity and assess any coexisting pelvic endometriotic lesions. Histological examination confirmed the diagnosis of umbilical endometriosis. Conclusion …

Adultendometriosismedicine.medical_specialtyUmbilicus (mollusc)primary umbilical endometriosisUmbilicuEndometriosislaparoscopyumbilical endometriomalcsh:Gynecology and obstetricsPathogenesisRare DiseasesObstetrics and GynaecologyMedicineHumansEndometriosiLaparoscopylcsh:RG1-991Histological examinationAbdominopelvic cavitymedicine.diagnostic_testbusiness.industryEndometriosis; Laparoscopy; Primary umbilical endometriosis; Umbilical endometrioma; Umbilicus; Obstetrics and GynecologyObstetrics and GynecologyPrimary umbilical endometriosimedicine.diseaseSurgeryumbilicusFemaleUmbilical bleedingbusinessAbdominal surgeryTaiwanese Journal of Obstetrics and Gynecology
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Isolated Endometriosis on the Rectus Abdominis Muscle in Women without a History of Abdominal Surgery: a Rare and Intriguing Finding

2009

We report 2 rare cases of endometriosis on the rectus abdominal muscle diagnosed incidentally during an operation for inguinal hernia repair in women with no surgical history. Two women sought medical attention for a mass found in the pubic abdominal wall. Only 1 woman reported occasional pain. At physical examination in both women, an ovoid swelling in the right pubic area was felt. One woman experienced pain on palpation, and one reported slight discomfort. Ultrasonography demonstrated a heterogeneous hypoechogenic formation with indistinct edges; diagnosis was difficult. Routine clinical and instrumental (pelvic ultrasonography) gynecologic examination in both patients performed shortly …

Adultmedicine.medical_specialtyAbdominal painAbdominal painRectus abdominal muscle endometriosisHerniaEndometriosisRectus AbdominisEndometriosisHernia InguinalAbdominal wall endometriosisPalpationAbdominal pain; Abdominal wall endometriosis; Rectus abdominal muscle endometriosis; Adult; Endometriosis; Female; Hernia Inguinal; Humans; Incidental Findings; Middle Aged; Rectus Abdominis; Ultrasonography; Diagnostic ErrorsAbdominal wallmedicineHumansDiagnostic ErrorsRectus abdominis musclePelvisUltrasonographyIncidental Findingsmedicine.diagnostic_testbusiness.industryObstetrics and GynecologyMiddle Agedmedicine.diseaseAbdominal wall endometriosiSettore MED/40 - Ginecologia E OstetriciaSurgeryInguinal herniamedicine.anatomical_structureabdominal pain; abdominal wall endometriosis; rectus abdominal muscle endometriosisInguinalFemalemedicine.symptombusinessAbdominal surgery
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